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Click "Continue" to proceed to sigmaaldrich. How to retrieve a certificate of analysis Use your Material and Lot Number to obtain Certificate of Analysis for life science Products. For general laboratory use. Product No. Pack Size purifications. Contact Us. Overview Application Performance Details Documents. Remove inhibitors that interfere with downstream assays, ensuring greater assay specificity, sensitivity, and reproducibility. Prepare RNA samples in only 20 minutes. Obtain concentrated RNA that is suitable for downstream applications.
Minimize RNA loss with a kit that removes contaminants without precipitation or other handling steps that degrade RNA. Eliminate the use of hazardous organic compounds such as cesium chloride, phenol, chloroform, and ethidium bromide. Your paper s will be evaluated for placement on lifescience.
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To order these products, please contact your Roche order management team. Other sample materials, such as whole blood, yeast, and bacteria require a pre-lysis treatment. The isolated RNA can be used in many downstream applications:. RNA was isolated from six identical samples, each containing 10 6 K human lymphocyte cells.
Four samples were treated with DNase as described in the Instructions for Use. For two samples, the DNase step was omitted. Right Panel: In a control reaction, amplification was performed without prior first strand cDNA synthesis. Result: After first strand cDNA synthesis, the expected fragment could be amplified from all samples left panel.
However, in the samples that were not treated with DNase, a fragment was also amplified in the control no cDNA synthesis reactions, indicating that those samples contained residual genomic DNA right panel; lanes 6 and 7. RNA was isolated from 10 6 K human lymphocyte cells with each kit, and yield was determined spectrophotometrically.
The indicated amounts of RNA from each isolate were separated on a denaturing formaldehyde gel and subjected to northern blotting. Nucleic acids bind to the surface of the glass fiber fleece in the presence of a chaotropic salt guanidine HCl. A single reagent which contains a chaotropic salt and detergent lyses the sample and simultaneously inactivates RNases.
After cell debris is removed by centrifugation, the lysate is applied to the glass fiber fleece in a High Pure Spin Filter Tube.
Under the buffer conditions used in the procedure, nucleic acids bind to the glass fiber fleece, while contaminating substances salts, proteins, and other cellular contaminants do not. Brief wash-and-spin steps readily remove the digested DNA fragments and other contaminating substances. Once purified, the RNA can be easily eluted in a small volume of low-salt buffer. RNA yield is determined by measuring the optical density at nm. Integrity and size distribution are examined by the banding pattern of ribosomal RNA in a denaturing agarose gel.
All kit components are function tested for absence of RNases. Online Technical Support Find help. Last Update On Products are for life science research only. Not for use in diagnostic procedures unless otherwise indicated.
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